Laboratory Report

DISCUSSION Microorganism atomic number 18 organism that argon too small and cannot be seen with naked eyes. The phrase of ubiquity of microorganism refers to the concept that microorganism are everywhere in our daily life surrounding. In our everyday common life ,microbes are virtually ubiquitous. They are in the air we breath,the foods we eat and as intimately as the skin of our fingers. Aseptic transfer is the transference of bacteria or other microbial cultures fromone container to another while maintaining purity of the culture. Pure culturesconsistof precisely one type of bacteria ideally the descendants from a item-by-item bacterial cell.Because microbes are present everywhere in the air, the work area, clothes, bodies,etc. , it is pregnant to follow the rules for aseptic transfer at all times. This is the onlyway of controlling pollutionMaintaining purity of culture is essential in microbiology if the biologist is to beable to identify bacteria, outpouring for antib iotic sensitivity, or maintain stock cultures. Oftenin nature a pure culture is impossible to come by because species live together. Thescientist is left functional with mixed cultures.Pure cultures can be derived from mixedcultures through isolation of cultures and this also requires that unimpregnated (aseptic)techniques to be apply. Normally transference is done from colonies. A colony consists of commonlyseveral meg cells that are assumed to be the descendants from one cell. Inoculations from one media to another, therefore, is usually done by removal of a fewmillion cells from one colony into a new environment. This must be done with theintegrity of all colonies remaining intact. by means of the use of sterile techniques, this canbe accomplished successfully.There are a number of tools that are utilise for inoculation procedures. Inoculating loops are used when transferring members of a gunstock culture to another broth, platedmedia or an agar slant. Inoculating needl es-are used when inoculating a broth culturefrom a colony on plated media or when reservation a stab in an agar deep or agar slantfrom broth or solid media. Forceps -are used to place sterile disks containing sometesting cistron in a broth culture or on a solid media culture. Pipets-are used when transferring liquids into other liquids or onto solid media.Flaming-is used to go up any microbes left on loops and needles. Alcohol flame-is used to sterilize forceps. When flaming inoculating loops and needles, careshould be taken avoid burning the plastic bring off at the end of each. The coat of theloop or needle should glow bolshy hot and and then be allowed to cool before dipping it into any cultures if the metal is too hot it testament kill the organisms that are to be used for inoculation. Alcohol flaming for the forceps is done by dipping the forceps into a smallamount of alcohol and then burning the alcohol off.The forceps should be dipped andburned three times. Care sho uld be taken to avoid alcohol running up toward the hand. The flame will follow the alcohol and burns will result. Pipets normally used in lab are prepackaged, sterile, disposable pipets. Sometimes glass pipets are used and these are stored in cans. The glass pipets arediscarded into a pipet jar make full with disinfectant. Disposable pipets are deposited inbiohazard bags. It is important that pipettors are always used and pipetting by mouth isprohibited.